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Types of DNA Analysis

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By Monte Wayne Miller

A process of conducting a study about genetic sequences, DNA-analysis is being used by medical personnel and law enforcement agencies to identify a particular species or person. It can even be used to determine specific diseases and cancers. At one point, this process becomes very limited. However,it is now analyzed using numerous processes.

The forensic DNA analysis comes in three different types: Y-Chromosome, STR or Short Tandem Repeats (uses PCR or Polymerase Chain Reaction), and mtDNA or Mitochondrial DNA.

Y-Chromosome Analysis

The Y chromosome is very useful in forensic applications as it helps identify the suspects. This chromosome can only be found in males and can only be passed down from a father to a son. Hence, analyzing this chromosome separates the female DNA and identifies familial relationships. Another advantage it offers is its ability to limit the pool of potential suspects even if there are numerous male contributors found in the biological sample. With the latest advancements, the process of extraction that is used to separate the vaginal cells from the semen prior to the DNA analysis becomes unnecessary.

Short Tandem Repeat or STR Analysis

Short Tandem Repeat or STR analysis examines the particular areas or loci in the nuclear or normal DNA. Each person comes with a distinct region of DNA. This process improves the difference of one DNA profile from the others. In cases of identical twins, the chances of two people sharing similar 15-loci profile is still one in a 1,000,000,000.

The FBI (Federal Bureau of Investigation) set a standard of 13 STR loci for the STR analysis and for DNA databases. This is to ensure that all forensic labs will have the ability to share and use the same forensic information. The CODIS database for unsolved cases creates profiles using the STR process, according to the 13 STR loci guideline set by the FBI.

Mitochondrial DNA-Analysis

The mtDNA or Mitochondrial analysis is used to examine samples that cannot be analyzed through an STR analysis due to non-availability of a nucleic DNA material. This type of uses a DNA that is taken out from the mitochondrion. Unlike the STR analysis that uses the cell’s nucleic DNA, the mtDNA extracts DNA from old sample evidence such as bones, teeth, and hairs that no longer have any nucleic DNA material. The mtDNA is an extremely beneficial process to help solve unidentified remains and cold cases.

Though the STR analysis can also be used in creating profiles from an old evidence, but the sample materials like semen or blood are sometimes destroyed in the PCR testing while generating an accurate profile. Nonetheless, the Mitochondrial DNA can be used to examine an old sample material like a hair shaft and eventually helps solve the case. Furthermore, the mitochondrial is only passed down from the mother to all of her offspring. Hence, everyone related to the mother has the same mitochondrial. Subsequently, this type of DNA-analysis helps determine any maternal relative of an unidentified remain.

Although this technique of identifying a suspect using the person’s genetic blueprint has been around since 1980s, DNA analysis has recently grown in popularity. TV shows have increased public awareness about analysis and forensics. So convincing and intriguing are the court cases that today most jurors would expect to see the DNA results in the courtroom. Moreover, analysis is undeniably the most important innovation in the police work next to the fingerprint analysis. This advancement in the genetic technology has brought many criminals to justice and helps solve not only the present-day cases, but also those that have been unsolved for many years.

In a nutshell, analysis has changed the society in different ways. With the use of the latest cutting-edge technology, this genetic analysis provides highly accurate results, which makes it highly trusted and used all over the world. Indeed, analysis has permeated almost all strata of the human society today.

Dr. Miller has over 14 years experience in the lab and as a crime scene investigator. He is experienced with DNA, evidence and stain collection and testing in sexual assaults, murders, and paternity.

Read more: Types of DNA Analysis http://www.sooperarticles.com/health-fitness-articles/genetics-articles/types-dna-analysis-1112211.html#ixzz4J7cVslNU
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